Many of them are always recorded with your data, like the cytometer you used ($CYT), the date the fle was acquired ($DATE), and the start time and end time ($BTIM and $ETIM, respectively). Keywords are important and can tell you a substantial amount of information about your fles. Maybe it doesn t bother you as much but, if you want to change it, you can. I prefer to see the gated population frst. I don t like how VX changes the parameters I m looking at when I view a child population. Keep the parameters of your child gates the same as your parent gate. Hold down Alt when you right-click on the color box of a legend to change all layers at once. Use line weight to make graph outlines thicker. Right-click on a parameter in the layout editor to change the axis quickly. Hold Alt and click a graph in the layout editor to duplicate it. Click on the page break intersections to change page size 35. Type in the name field for any statistics in the table editor to give that column a custom name. Create correlation and line plots in the table editor. Know when to use mean, geometric mean, or median. Drag an unstained control to use as a universal negative in compensation. Use the phrase comp in your compensation controls. Hold down Alt when you click to collapse it will collapse everything. Explore your data with multigraph overlays. Use ancestry and back gating to get your point across quickly. Add a text box to embed keywords, and even statistics, right into your figure. Make sure your axes are labeled properly and cleanly with something that makes sense. If you only have a few events, use the option to show large dots. Use the plus and minus signs to quickly increase and decrease the gate size. 1 Hacking FlowJo VX 42 Time-Saving FlowJo Shortcuts To Help You Get Your Data Published No Matter What Flow Cytometer It Came FromĢ CONTENTS 1.
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